Synchronization of Zygote Production in Saccharomyces cerevisiae

Mitochondrial genetic analysis by zygote cell lineages in Saccharomyces cerevisiae.

Yeast strains were constructed carrying multiple mitochondrial markers conferring resistance to the inhibitors erythromycin, chloramphenicol, paromomycin and oligomycin. A pedigree analysis of two crosses was made by micromanipulating buds from zygotes. The first few daughter buds isolated from the zygotes sometimes gave rise to diploid clones which had a mixture of mitochondrial types. All pos...

Tubulin and actin topology during zygote formation of Saccharomyces cerevisiae.

The topology of tubulin and actin during mating of Saccharomyces cerevisiae was analysed by fluorescence microscopy with the monoclonal anti-tubulin antibody Tu01 and rhodamine-labelled phalloidin. Preconjugatory cells displayed an asymmetric distribution of the microtubule and actin cytoskeleton and an overall polarization of the cells preceding cell fusion. Prior to karyogamy, the haploid spi...

Production of Single Cell Protein from Sugarcane Bagasse by Saccharomyces cerevisiae in Tray Bioreactor

In this study, solid state fermentation (SSF) was carried out to produce single cell protein (SCP) from sugarcane bagasse using Saccharomyces cerevisiae. The SSF experiment were performed in a tray bioreactor. The influence of several parameters including extraction buffer, initial moisture content of substrate, fermentation time, relative humidity in bioreactor, the bioreactor temperature and ...

Disruption of GAL1 Gene in Saccharomyces cerevisiae Leads to Higher Ethanol Production

Characterization of Encapsulated Berberine in Yeast Cells of Saccharomyces cerevisiae

Berberine was loaded in yeast cells of Saccharomyces cerevisiaeas a novel pharmaceutical carrier to improve the treatment ofmany diseases. The yeast-encapsulated active materialsshowedhigh stability and bioavailability due to the enhanced solubility and sustained releasing. In this study, different characteristics of prepared berberine loaded yeast cells (loading capacity, release kinetic order...